All of the electroporation technology has utilized the electroporation cuvettes as the disposable electric chambers for the delivery of high electric field to the biological samples. These electroporation cuvettes are composed of plastic chamber with narrow gap (0.2 ~ 0.4 cm gap) between two plate-type electrodes. However, the scientists at the Digital Bio Technology has found that most of the low transfection efficiencies are originated from this cuvette design.

The core technology of MICRIPORATION is utilization of capillary instead of the cuvette. In the capillary type of electric chamber, the gap size between two electrode is maximized and the surface area of electrode can be minimized compared to the cuvette type chamber. By doing so, the transfection efficiency and cell viability is dramatically increased. Why Microporation shows an outstanding transfection efficiency? Although the basic mechanism of microporation technology has not been elucidated well, it is evident that the increment of the gap size between two electrode shows an increment of transfection efficiency, mostly by the uniform electric field generated in the long and narrow capillary.

The harmful effects of large electrode surface area has been well known. For example, water dissociation during electroporation procedure generates O2 and H2 at each electrode. Also metal ion can be dissolved in the samples during electroporation. By these chemical reaction, harmful metal oxides are formed and pH is decreased. High heat generation is another harmful effect of conventional electroporation. However, Microporation technology eliminates all these problems of conventional electroporation, since the electrode surface area can be minimized in the capillary type electroporation chamber. Minimal pH decrement and metal ion formation, negligible heat generation is one of the most successful outcome of Microporation technology.

 Microporation of GFP plasmid

Never existed High Transfection Efficiency!  Both for cell lines and primary cells

PC-12, Jurkat, HL-60, HaCaT cells are difficult to transfect with conventional methods, e.g. lipid-based reagents and other electroporator. But, based on a novel physical principle, Microporation shows an excellent transfection efficiency and high cell viability. Simple electroporation protocol has never been compromised with the transfection efficiency.

 Transfection efficiency by Microporator

Transfection of hard-to-transfect cell lines by Microporator

Cells were transfected using the Microporator and 0.5 ug of a plasmid encoding the EGFP were used.
24 hours post microporation, the cells were analyzed by light and fluorescence Microscopy.

 Transfection efficiency by Microporator

Microporation of siRNA

pEGFP and each siRNA were co-transfected by Microporation. GFP expressing cells were counted under fluorescent microscope after 48 hours

Microporation of siRNA

Microporation of 3T3-L1 ad

3T3-L1 (differentiated adipocyte) cells were microporated using the Microporator and 0.5 ug of pEGFP-N1 plasmid. 24 hours post microporation the cells were analyzed by fluorescence microscopy.

 (GFP transfection efficiency = 50%, Viability = 80%)

Microporation for Promoter

Microporation is a very efficient transfection tool for promoter study. In terms of sensitivity, only 1 ng transfected pGL3-control vector resulted in high R.L.U value from luciferase gene assay (Fig B). In terms of reliability, Microporation consistently reflects the activity of various promoters (Fig A).

A. Transfection of various promoter-luciferase constructs with Microporation

B. Sensitivity of reporter gene assay combined with Microporation

1. Jun Geun Chang, Keunchang Cho, Jeong Ah Kim, and Chanil Chung “An     Electroporation
    Device Comprising a Tube or a Capillary” Kor. Patent No. 2004-88245 (2004).

2. Jun Geun Chang, Chanil Chung, Keunchang Cho, Young Shik Shin, Jeong Ah Kim,
    and Youn Chul Jung “Electroporator Having an Elongated Hollow Member”
    PCT/KR2005/001792 (2005).

Brochure Download

Microporator Catalog.pdf